Tal T, Aviram I
Department of Biochemistry, Faculty of Life Sciences, Tel-Aviv University, Israel.
Biochem Biophys Res Commun. 1993 Oct 29;196(2):636-41. doi: 10.1006/bbrc.1993.2297.
HNP-1 [correction of NHP-1], a member of a family of microbicidal cationic, cystine-rich polypeptides abundant in the azurophilic granules of polymorphonuclear leukocytes (PMN) interfered with detergent-induced cell-free activation of superoxide-producing NADPH oxidase. IC50 of 3 microM has been evaluated for this dose dependent effect of defensin. Preactivated oxidase was not affected by HNP-1. The inhibitory effect could be relieved by raising the concentration of the cytosol or by the addition of a partially purified p47-phox. NADPH oxidase activated in the presence of HNP-1 exhibited an increased affinity for NADPH.
