Chen F, Law S W, O'Malley B W
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.
Biochem Biophys Res Commun. 1993 Oct 29;196(2):671-7. doi: 10.1006/bbrc.1993.2302.
Two additional forms of mouse peroxisome proliferator activated receptor have been identified from cDNA libraries derived from mouse tissues using a low stringency cross-hybridization screening method. One is mNUCI, which has 97% amino acid sequence identity with hNUCI in its DNA binding domain. The other one is mPPAR gamma. The full-length cDNA of mPPAR gamma encodes a protein of 470 amino acids in length. The overall amino acid sequence identity is 75% as compared with xPPAR gamma, with the highest homology in the DNA binding region and ligand binding region, 97% and 86% identity, respectively. The discovery of two new forms of mPPAR and the recent cloning of hPPAR provide evidence for the existence of at least five different forms of receptor in the peroxisome proliferator activated receptor subfamily.
利用低严格度交叉杂交筛选方法，从小鼠组织来源的cDNA文库中鉴定出另外两种形式的小鼠过氧化物酶体增殖物激活受体。一种是mNUCI，其在DNA结合结构域与hNUCI具有97%的氨基酸序列同一性。另一种是mPPARγ。mPPARγ的全长cDNA编码一个长度为470个氨基酸的蛋白质。与xPPARγ相比，整体氨基酸序列同一性为75%，在DNA结合区域和配体结合区域具有最高同源性，分别为97%和86%的同一性。两种新形式的mPPAR的发现以及最近hPPAR的克隆为过氧化物酶体增殖物激活受体亚家族中至少存在五种不同形式的受体提供了证据。
