Identification of two mPPAR related receptors and evidence for the existence of five subfamily members.

Two additional forms of mouse peroxisome proliferator activated receptor have been identified from cDNA libraries derived from mouse tissues using a low stringency cross-hybridization screening method. One is mNUCI, which has 97% amino acid sequence identity with hNUCI in its DNA binding domain. The other one is mPPAR gamma. The full-length cDNA of mPPAR gamma encodes a protein of 470 amino acids in length. The overall amino acid sequence identity is 75% as compared with xPPAR gamma, with the highest homology in the DNA binding region and ligand binding region, 97% and 86% identity, respectively. The discovery of two new forms of mPPAR and the recent cloning of hPPAR provide evidence for the existence of at least five different forms of receptor in the peroxisome proliferator activated receptor subfamily.