Brenner D J, Grimont P A, Steigerwalt A G, Fanning G R, Ageron E, Riddle C F
Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
Int J Syst Bacteriol. 1993 Oct;43(4):645-58. doi: 10.1099/00207713-43-4-645.
DNA relatedness studies (hydroxyapatite method) were done on 112 strains of citrobacteria. By using the recommended definition of a genomospecies 11 genomospecies were identified in the genus Citrobacter. These genomospecies were separable by their biochemical profiles. Citrobacter koseri (Citrobacter diversus) and Citrobacter amalonaticus proved to be homogeneous species, as previously described. C. amalonaticus biogroup 1, as described by Farmer et al. (J. Clin. Microbiol. 21:46-76, 1985), was shown to be a separate homogeneous species, which was named Citrobacter farmeri sp. nov. The Citrobacter freundii complex was quite heterogeneous. C. freundii sensu stricto, as represented by the type strain, contained only 9 of 66 strains in this complex. The remaining 57 strains were members of seven genomospecies. Genomospecies 5, containing 21 strains, was named Citrobacter youngae sp. nov. Genomospecies 6, containing 15 strains, was named Citrobacter braakii sp. nov. Genomospecies 7 and 8, each containing six strains, were named Citrobacter werkmanii sp. nov. and Citrobacter sedlakii sp. nov., respectively. Genomospecies 9, 10, and 11, each containing three strains, were not named.
对112株柠檬酸杆菌进行了DNA相关性研究（羟基磷灰石法）。通过使用基因组种的推荐定义，在柠檬酸杆菌属中鉴定出11个基因组种。这些基因组种可通过其生化特征区分。如先前所述，科氏柠檬酸杆菌（奇异柠檬酸杆菌）和无丙二酸柠檬酸杆菌被证明是同源种。如Farmer等人（《临床微生物学杂志》21:46 - 76，1985）所描述的无丙二酸柠檬酸杆菌生物群1，被证明是一个单独的同源种，命名为法氏柠檬酸杆菌新种。弗氏柠檬酸杆菌复合体相当异质。以模式菌株为代表的严格意义上的弗氏柠檬酸杆菌，在该复合体的66株菌株中仅包含9株。其余57株是7个基因组种的成员。包含21株菌株的基因组种5，命名为杨氏柠檬酸杆菌新种。包含15株菌株的基因组种6，命名为布拉氏柠檬酸杆菌新种。分别包含6株菌株的基因组种7和8，分别命名为韦氏柠檬酸杆菌新种和塞氏柠檬酸杆菌新种。分别包含3株菌株的基因组种9、10和11未命名。
