Janda J M, Abbott S L, Cheung W K, Hanson D F
Microbial Diseases Laboratory, Department of Health Services, Berkeley, California 94704-1011.
J Clin Microbiol. 1994 Aug;32(8):1850-4. doi: 10.1128/jcm.32.8.1850-1854.1994.
We biochemically identified 235 Citrobacter strains to the species level on the basis of the recently proposed taxonomic changes of Brenner et al. (D. J. Brenner, P. A. D. Grimont, A. G. Steigerwalt, G. R. Fanning, E. Ageron, and C. F. Riddle, Int. J. Syst. Bacteriol. 43:645-658, 1993). Citrobacter isolates were initially identified as C. koseri or as members of the C. freundii complex or C. amalonaticus group on the basis of indole production, formation of H2S, malonate utilization, and acid production from D-arabitol and adonitol. On the basis of the results of these tests, 68% of the Citrobacter strains were identified as members of the C. freundii complex, 25% were C. koseri, and 8% were members of the C. amalonaticus group. By using a 15-test system recently proposed by Brenner et al. (D. J. Brenner, P. A. D. Grimont, A. G. Steigerwalt, G. R. Fanning, E. Ageron, and C. F. Riddle, Int. J. Syst. Bacteriol. 43:645-658, 1993) to help identify new species in the C. freundii complex and C. amalonaticus group, 81% of the C. freundii complex strains and 100% of the C. amalonaticus strains could be definitively assigned to one of the previously established or recently designated species or hybridization groups of the genus Citrobacter. Within the C. freundii complex, C. freundii predominated overall (37%), followed by C. youngae (24%), C. braakii (13%), and C. werkmanii (6%). Only one strain each of C. sedlakii and Citrobacter DNA group 11 was identified in this study. Among C. amalonaticus complex members, all were identified as C. amalonaticus with the singular exception of one fecal isolate of C. farmeri. C. freundii and C. koseri were the two Citrobacter species most commonly (80 of 93 [86%]) isolated from extraintestinal sources (genitourinary tract, wounds, blood).
我们根据布伦纳等人最近提出的分类学变化（D. J. 布伦纳、P. A. D. 格里蒙、A. G. 施泰格瓦尔特、G. R. 范宁、E. 阿热龙和C. F. 里德尔，《国际系统细菌学杂志》43:645 - 658，1993年），从生化角度将235株柠檬酸杆菌鉴定到种水平。柠檬酸杆菌分离株最初根据吲哚产生、H2S形成、丙二酸利用以及从D - 阿拉伯糖醇和阿东糖醇产酸情况，被鉴定为科氏柠檬酸杆菌或弗氏柠檬酸杆菌复合体成员或无丙二酸柠檬酸杆菌群成员。根据这些测试结果，68%的柠檬酸杆菌菌株被鉴定为弗氏柠檬酸杆菌复合体成员，25%为科氏柠檬酸杆菌，8%为无丙二酸柠檬酸杆菌群成员。通过使用布伦纳等人最近提出的一个包含15项测试的系统（D. J. 布伦纳、P. A. D. 格里蒙、A. G. 施泰格瓦尔特、G. R. 范宁、E. 阿热龙和C. F. 里德尔，《国际系统细菌学杂志》43:645 - 658，1993年）来帮助鉴定弗氏柠檬酸杆菌复合体和无丙二酸柠檬酸杆菌群中的新物种，81%的弗氏柠檬酸杆菌复合体菌株和100%的无丙二酸柠檬酸杆菌菌株能够被明确归为柠檬酸杆菌属先前已确立或最近指定的物种或杂交群之一。在弗氏柠檬酸杆菌复合体中，弗氏柠檬酸杆菌总体上占主导（37%），其次是杨氏柠檬酸杆菌（24%）、布氏柠檬酸杆菌（13%）和韦氏柠檬酸杆菌（6%）。在本研究中，仅鉴定出一株塞德拉克柠檬酸杆菌和一株柠檬酸杆菌DNA群11。在无丙二酸柠檬酸杆菌复合体成员中，除了一株法氏柠檬酸杆菌粪便分离株外，所有菌株均被鉴定为无丙二酸柠檬酸杆菌。弗氏柠檬酸杆菌和科氏柠檬酸杆菌是从肠道外来源（泌尿生殖道、伤口、血液）最常分离出的两种柠檬酸杆菌物种（93株中有80株[86%]）。
