Owen J J, Raff M C, Cooper M D
Eur J Immunol. 1976 Jul;5(7):468-73. doi: 10.1002/eji.1830050708.
Using an organ culture technique, the development of B lymphocytes has been studied in a number of isolated embryonic hemopoietic tissues. Developing B lymphocytes were identified by cytoplasmic and surface immunofluorescence using purified class-specific antibodies and a Fab anti-immunoglobulin reagent. In fetal liver cultures these cells were further characterized for Fc and complement receptors and for the presence of mouse-specific B lymphocyte antigen. The results indicate that B lymphocytes develop independently in fetal liver and in fetal spleen; they do not develop in cultures of yolk sac and thymus. In conclusion, it is likely that B lymphocyte maturation in mammals is multifocal and is not dependent upon gastrointestinal influences as suggested previously.
运用器官培养技术，已在多种分离的胚胎造血组织中研究了B淋巴细胞的发育。使用纯化的类特异性抗体和Fab抗免疫球蛋白试剂，通过细胞质和表面免疫荧光鉴定发育中的B淋巴细胞。在胎肝培养物中，对这些细胞进一步进行了Fc和补体受体以及小鼠特异性B淋巴细胞抗原存在情况的表征。结果表明，B淋巴细胞在胎肝和胎脾中独立发育；它们在卵黄囊和胸腺培养物中不发育。总之，哺乳动物中的B淋巴细胞成熟很可能是多灶性的，并不像先前认为的那样依赖于胃肠道的影响。
