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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Niimi T, Yamashita O, Yaginuma T

Laboratory of Sericultural Science, Faculty of Agriculture, Nagoya University, Japan.

Comp Biochem Physiol B. 1993 Oct;106(2):437-42. doi: 10.1016/0305-0491(93)90326-z.

By using semi-quantitative PCR analysis, changes in the amount of the transcript for a Bombyx homolog of mammalian sorbitol dehydrogenase (BmSDH) were examined during embryogenesis in non-diapause eggs. 2. Occurrence of the transcript for BmSDH correlated with the two developmental phases, the growth of embryo and the formation of larval tissues. 3. In the first phase, an increase in the amount of the transcript for BmSDH resulted from embryonic cells rather than yolk cells. 4. In the second phase, the transcript was suggested to be abundant in fat-body cells of pharate larva, because it was abundant in fat-bodies of 5th instar larvae. 5. The difference in the expression of BmSDH gene between diapause and non-diapause eggs is discussed.

通过半定量PCR分析，检测了非滞育卵胚胎发育过程中家蚕山梨醇脱氢酶（BmSDH）同源物转录本数量的变化。2. BmSDH转录本的出现与胚胎生长和幼虫组织形成这两个发育阶段相关。3. 在第一阶段，BmSDH转录本数量的增加源于胚胎细胞而非卵黄细胞。4. 在第二阶段，推测该转录本在即将孵化的幼虫脂肪体细胞中含量丰富，因为其在五龄幼虫的脂肪体中含量丰富。5. 讨论了滞育卵和非滞育卵之间BmSDH基因表达的差异。

Niimi T, Yamashita O, Yaginuma T

Laboratory of Sericultural Science, Faculty of Agriculture, Nagoya University, Japan.

Comp Biochem Physiol B. 1993 Oct;106(2):437-42. doi: 10.1016/0305-0491(93)90326-z.

By using semi-quantitative PCR analysis, changes in the amount of the transcript for a Bombyx homolog of mammalian sorbitol dehydrogenase (BmSDH) were examined during embryogenesis in non-diapause eggs. 2. Occurrence of the transcript for BmSDH correlated with the two developmental phases, the growth of embryo and the formation of larval tissues. 3. In the first phase, an increase in the amount of the transcript for BmSDH resulted from embryonic cells rather than yolk cells. 4. In the second phase, the transcript was suggested to be abundant in fat-body cells of pharate larva, because it was abundant in fat-bodies of 5th instar larvae. 5. The difference in the expression of BmSDH gene between diapause and non-diapause eggs is discussed.

通过半定量PCR分析，检测了非滞育卵胚胎发育过程中家蚕山梨醇脱氢酶（BmSDH）同源物转录本数量的变化。2. BmSDH转录本的出现与胚胎生长和幼虫组织形成这两个发育阶段相关。3. 在第一阶段，BmSDH转录本数量的增加源于胚胎细胞而非卵黄细胞。4. 在第二阶段，推测该转录本在即将孵化的幼虫脂肪体细胞中含量丰富，因为其在五龄幼虫的脂肪体中含量丰富。5. 讨论了滞育卵和非滞育卵之间BmSDH基因表达的差异。