Genomics Division, Seribiotech Research Laboratory, Carmelaram Post, Kodathi, Bangalore, 560 035, India.
Genomics. 2013 Oct;102(4):379-87. doi: 10.1016/j.ygeno.2013.07.007. Epub 2013 Jul 23.
Diapause was induced in polyvoltine silkworm B. mori eggs and the molecular mechanism involved in diapause was investigated using a genome wide microarray. In diapause eggs, 638 and 675 genes were upregulated, while, in non-diapause eggs 1136 and 595 genes were upregulated at 18 h and 30 h, respectively after oviposition. Real-time qPCR analysis confirmed the expression of 20 genes, and the relative expression levels of the Aquaporin gene was highest among the 20 genes, followed by Sorbitol dehydrogenase-2 and Cytochrome b5 in diapause eggs, while, Kruppel homolog, Period and Relish were higher in non-diapause eggs. The upregulation of SDH-2 and cytochrome b5 indicates increased metabolic rate in diapause-destined embryos prior to the onset of diapause within 36 h as a preparatory phase. This study provides an insight into the early molecular events for the induction and maintenance of diapause in B. mori.
多化性桑蚕 B. mori 卵被诱导滞育，使用全基因组微阵列研究了涉及滞育的分子机制。在滞育卵中，分别在产卵后 18 小时和 30 小时，有 638 个和 675 个基因上调，而在非滞育卵中，有 1136 个和 595 个基因上调。实时 qPCR 分析证实了 20 个基因的表达，其中 Aquaporin 基因的相对表达水平最高，其次是 Sorbitol dehydrogenase-2 和 Cytochrome b5 在滞育卵中，而 Kruppel homolog、Period 和 Relish 在非滞育卵中更高。SDH-2 和细胞色素 b5 的上调表明在滞育开始前的 36 小时内，滞育定向胚胎的代谢率升高，作为预备阶段。本研究深入了解了 B. mori 中滞育的诱导和维持的早期分子事件。
