Zalensky A O, Breneman J W, Zalenskaya I A, Brinkley B R, Bradbury E M
Department of Biological Chemistry, School of Medicine, University of California, Davis 95616.
Chromosoma. 1993 Sep;102(8):509-18. doi: 10.1007/BF00368344.
The localization of centromeres in mature human sperm was shown by immunofluorescent labeling and nonisotopic in situ hybridization. In the decondensed nucleus structural elements (dimers, tetramers, linear arrays and V shape structures) formed by individual centromeres of nonhomologous chromosomes were observed. They organize the compact chromocenter, which was shown for nuclei decondensed to a low extent. The chromocenter is buried inside the nucleus; in contrast, telomeric regions of chromosomes were tentatively localized on the periphery. Thus, a gross architecture, which can influence selective unpackaging of the paternal genome upon fertilization, exists in human sperm.
通过免疫荧光标记和非同位素原位杂交显示了成熟人类精子中着丝粒的定位。在解聚的细胞核中，观察到由非同源染色体的单个着丝粒形成的结构元件（二聚体、四聚体、线性阵列和V形结构）。它们组成了紧密的染色中心，这在低程度解聚的细胞核中得到了证实。染色中心深埋在细胞核内部；相比之下，染色体的端粒区域初步定位在细胞核周边。因此，人类精子中存在一种总体结构，它可能会影响受精时父本基因组的选择性解包装。
