Eriksson N P, Persson J K, Svensson M, Arvidsson J, Molander C, Aldskogius H
Department of Anatomy, Karolinska Institutet, Stockholm, Sweden.
Exp Brain Res. 1993;96(1):19-27. doi: 10.1007/BF00230435.
The time course of the microglial cell reaction in central nervous system primary sensory projection territories has been examined following peripheral nerve injury in the adult rat using qualitative and quantitative analysis of immunoreactivity with the monoclonal antibody OX-42, which recognises the complement receptor CR3. The regions examined included the gracile nucleus, the column of Clarke and the spinal cord dorsal horn (superficial and deep laminae separately) after unilateral sciatic nerve transection, and the spinal trigeminal nucleus following unilateral infraorbital nerve transection. In all territories examined a qualitative increase in OX-42 immunoreactivity was observed 24 h postlesion. Further, quantitative analysis revealed an exponential development of the OX-42 immunoreactivity, with a peak at one week postlesion, thereafter showing a slow exponential decline. Our results show that the signal (or signals) that induces the microglial cell response in primary sensory projection territories is rapid in comparison to previously described central degenerative changes following peripheral nerve lesions (transganglionic degeneration). These findings are compatible with the hypothesis that activated microglia play a pathogenetic role in the development of transganglionic degeneration.
利用针对识别补体受体CR3的单克隆抗体OX-42的免疫反应性进行定性和定量分析，研究了成年大鼠外周神经损伤后中枢神经系统初级感觉投射区域内小胶质细胞反应的时间进程。所检查的区域包括单侧坐骨神经横断后薄束核、克拉克柱和脊髓背角（分别为浅、深层板层），以及单侧眶下神经横断后的三叉神经脊束核。在所有检查区域，损伤后24小时观察到OX-42免疫反应性出现定性增加。此外，定量分析显示OX-42免疫反应性呈指数发展，在损伤后一周达到峰值，此后呈缓慢指数下降。我们的结果表明，与先前描述的外周神经损伤（跨神经节变性）后的中枢退行性变化相比，在初级感觉投射区域诱导小胶质细胞反应的信号是快速的。这些发现与激活的小胶质细胞在跨神经节变性发展中起致病作用的假设一致。
