Giannarelli S, Balas D, Rampal P
Laboratoire de Gastroentérologie et de Nutrition, Faculté de Médecine, Nice.
Gastroenterol Clin Biol. 1993;17(6-7):471-7.
Gross normal specimens of human distal colon were obtained at operation for cancer. Smooth muscle cells were separated from internal and external layers of the muscularis. They were dissociated by digestion with collagenase, isolated and concentrated by successive centrifugations. Colonic smooth muscle cell contraction was measured using various concentrations of carbamylcholine (10(-9) to 10(-4) M); relaxation was tested using atropine (10(-9) to 10(-4) M) on colonic smooth muscle cells pre-contracted by carbamylcholine. Compared with previous descriptions, human smooth muscle cells were smaller than in other species with an enlarged distribution of cell size (30 microns to 150 microns in length). Significant dose-response curves were obtained for both carbamylcholine and atropine. However, 3 original points characterized human colonic smooth muscle cells: a) the cells isolated from the internal layer were significantly more sensitive than those isolated from the external layer (10(-9) M vs 10(-7) M); b) for the muscle cells isolated from both the internal and external layers, small colonic smooth muscle cells were significantly more sensitive. On the other hand, these cells were shown to be located near conjunctive septae, and intramural plexuses; c) analysis of contraction curves demonstrated a more efficient response for colonic smooth muscle cells of the internal layer than for those of the external layer of the muscularis.
在癌症手术中获取了正常的人类远端结肠大体标本。从肌层的内层和外层分离出平滑肌细胞。通过用胶原酶消化使其解离，经连续离心分离并浓缩。使用不同浓度的氨甲酰胆碱（10⁻⁹至10⁻⁴M）测量结肠平滑肌细胞的收缩；在由氨甲酰胆碱预收缩的结肠平滑肌细胞上，使用阿托品（10⁻⁹至10⁻⁴M）测试舒张情况。与先前描述相比，人类平滑肌细胞比其他物种的小，细胞大小分布范围扩大（长度为30微米至150微米）。氨甲酰胆碱和阿托品均获得了显著的剂量反应曲线。然而，人类结肠平滑肌细胞有3个原始特征：a）从内层分离的细胞比从外层分离的细胞明显更敏感（10⁻⁹M对10⁻⁷M）；b）对于从内层和外层分离的肌细胞，小的结肠平滑肌细胞明显更敏感。另一方面，这些细胞显示位于结缔组织隔膜和壁内神经丛附近；c）收缩曲线分析表明，肌层内层的结肠平滑肌细胞比外层的结肠平滑肌细胞反应更有效。
