Parody T R, Muskavitch M A
Department of Biology, Indiana University, Bloomington 47405.
Genetics. 1993 Oct;135(2):527-39. doi: 10.1093/genetics/135.2.527.
Analysis of the development of Delta (Dl) temperature-sensitive mutants pulsed at restrictive temperature during larval and pupal stages reveals multiple phenocritical periods during which reduction of Dl function affects viability and development of adult structures. Dl function is required during the third larval instar for post-pupal viability and during the first day of pupal development for viability through eclosion. Dl function is required biphasically for the development of sensory bristles. Earlier pulses lead to bristle multiplication and later pulses lead to bristle loss. The exact intervals during which multiplication and loss are induced vary for different bristles. Dl function is also required for development of most, if not all, cell types in the retina. Different pulses result in reduction in eye size, scarring, and glossiness, as well as multiplication and loss of interommatidial bristles. We also define intervals during which Dl function is required for aspects of leg and wing development. Phenocritical periods for Dl function are temporally coincident with those previously reported for Notch (N), consistent with the hypothesis that the proteins encoded by Dl and N interact throughout development to assure correct specification of cell fates in a variety of imaginal tissues.
对在幼虫和蛹期的限制温度下进行脉冲处理的Delta（Dl）温度敏感突变体的发育分析表明，存在多个表型关键期，在此期间Dl功能的降低会影响成虫结构的活力和发育。在幼虫第三龄期，Dl功能是蛹后存活所必需的；在蛹发育的第一天，Dl功能是羽化存活所必需的。Dl功能对于感觉刚毛的发育具有双相需求。早期脉冲导致刚毛增殖，后期脉冲导致刚毛缺失。不同刚毛诱导增殖和缺失的确切间隔有所不同。视网膜中大多数（如果不是全部）细胞类型的发育也需要Dl功能。不同的脉冲会导致眼睛大小减小、瘢痕形成和光泽度降低，以及小眼间刚毛的增殖和缺失。我们还确定了腿部和翅膀发育方面需要Dl功能的间隔。Dl功能的表型关键期在时间上与先前报道的Notch（N）的关键期一致，这与以下假设一致：Dl和N编码的蛋白质在整个发育过程中相互作用，以确保各种成虫组织中细胞命运的正确特化。
