Mattheij J A, Swarts J J, van der Heijden A J, van Helvoort A L, Kusters I C
Department of Human and Animal Physiology, Agricultural University, Wageningen, The Netherlands.
Gynecol Obstet Invest. 1993;36(3):129-35. doi: 10.1159/000292611.
Fertile life of oocytes is usually considered to be related to ovulation time. In the present study, fertile life of rat oocytes was studied in relation to resumption of meiosis. In pro-oestrus, meiotic resumption without concomitant ovulation was induced in most graafian follicles by injection of a small amount of LH or FSH followed by Nembutal. These follicles either ovulated or developed into luteinized unruptured follicles if Ovalyse (a GnRH analogue) was given 8 h after LH or FSH. In subsequent experiments, rats were injected with FSH or saline, and Nembutal; 4 or 8 h later, Ovalyse was given to induce ovulation; the rats were mated 14 h after Ovalyse. At day 20 of pregnancy, fetal survival was 30% in rats with meiosis advanced by 8 h, against 91% and 70% in rats advanced by 0 or 4 h, respectively. Mortality occurred mainly during pre-implantation and early post-implantation. Advanced resumption of meiosis may cause pre-ovulatory ageing of oocytes; consequently, viability of these oocytes after ovulation is reduced.
卵母细胞的可育期通常被认为与排卵时间有关。在本研究中，研究了大鼠卵母细胞的可育期与减数分裂恢复的关系。在发情前期，通过注射少量促黄体生成素（LH）或促卵泡生成素（FSH），随后注射戊巴比妥，诱导大多数格拉夫卵泡进行减数分裂恢复但不伴随排卵。如果在LH或FSH注射8小时后给予奥维利斯（一种促性腺激素释放激素类似物），这些卵泡要么排卵，要么发育成黄素化未破裂卵泡。在随后的实验中，给大鼠注射FSH或生理盐水以及戊巴比妥；4或8小时后，给予奥维利斯诱导排卵；在奥维利斯注射14小时后让大鼠交配。在妊娠第20天，减数分裂提前8小时的大鼠胎儿存活率为30%，而减数分裂提前0或4小时的大鼠胎儿存活率分别为91%和70%。死亡主要发生在植入前和植入后早期。减数分裂的提前恢复可能导致卵母细胞排卵前老化；因此，这些卵母细胞排卵后的活力降低。
