Gimenez F, Dumartin C, Wainer I W, Farinotti R
Dosage de Médicaments, Service Pharmacie, Hôpital Pitié Salpêtriere, Paris, France.
J Chromatogr. 1993 Sep 8;619(1):161-6. doi: 10.1016/0378-4347(93)80461-c.
A liquid chromatographic method for the determination of the enantiomers of mefloquine has been improved. The chromatography involved two columns: an achiral cyanopropyl stationary phase for the quantification of (+/-)-mefloquine and a chiral naphthyl-urea stationary phase for the determination of the enantiomeric ratio. Compared with the previous method, which needed two detectors, this one used one detector-integrator to which the two columns are connected alternately by an automated column-switching system. The method is suitable for the quantification (0.05 microgram/ml) of mefloquine and the determination of enantiomeric ratios from 500-microliters plasma samples with ultraviolet detection.
一种测定甲氟喹对映体的液相色谱方法得到了改进。该色谱法使用两根色谱柱：一根非手性氰丙基固定相用于定量（±）-甲氟喹，一根手性萘基脲固定相用于测定对映体比例。与之前需要两个检测器的方法相比，该方法使用一个检测器积分仪，两根色谱柱通过自动柱切换系统交替连接到该检测器积分仪上。该方法适用于对甲氟喹进行定量（0.05微克/毫升），并通过紫外检测从500微升血浆样品中测定对映体比例。
