Gimenez F, Farinotti R, Thuillier A, Hazebroucq G, Wainer I W
Pharmaceutical Division, St. Jude Children's Research Hospital, Memphis, TN 38105.
J Chromatogr. 1990 Aug 3;529(2):339-46. doi: 10.1016/s0378-4347(00)83840-2.
A coupled achiral-chiral high-performance liquid chromatographic system has been developed for the determination of the enantiomers of mefloquine, (+)-MFQ and (-)-MFQ, in plasma and whole blood. The MFQ was separated from the interfering components in the biological matrix and quantified on a cyano-bonded phase, and the enantiomeric composition was determined on an (S)-naphthylurea chiral stationary phase. The two columns were connected by a switching valve equipped with a silica precolumn. The precolumn was used to concentrate the MFQ in the eluent from the achiral column before backflushing onto the chiral phase. The coupled-column system was validated and applied to the analysis of a pilot study of the pharmacokinetics of (+)- and (-)-MFQ in plasma and whole blood.
已开发出一种非手性-手性联用高效液相色谱系统，用于测定血浆和全血中盐酸甲氟喹（mefloquine，MFQ）对映体（+）-MFQ和（-）-MFQ。MFQ在生物基质中与干扰成分分离，并在氰基键合相上进行定量，对映体组成则在（S）-萘基脲手性固定相上测定。两根色谱柱通过配备硅胶预柱的切换阀相连。预柱用于在将洗脱液从非手性柱反冲到手性相之前浓缩其中的MFQ。该联用柱系统经过验证，并应用于一项关于（+）-和（-）-MFQ在血浆和全血中药代动力学的初步研究分析。
