Moore T D, Metcalf A C, Swagzdis J E, Doyle E
Department of Drug Metabolism and Pharmacokinetics, SmithKline Beecham Pharmaceuticals, Philadelphia, PA 17406.
J Chromatogr. 1993 Sep 8;619(1):172-6. doi: 10.1016/0378-4347(93)80463-e.
A reversed-phase high-performance liquid chromatographic (HPLC) assay using ultraviolet spectrophotometric detection has been developed for the determination of the concentration of 3-butyryl-4-(2-methylphenylamino)-8-(2-hydroxyethoxy)quinoline (I) in rat, dog and human plasma. Prior to analysis, the protein in plasma samples was precipitated with acetonitrile containing 3-butyryl-4-(2-methylphenylamino)-8-methoxyquinoline to act as an internal standard. The supernatant layer was injected onto the HPLC column with no further clean-up. The assay requires 200 microliters of plasma and is precise and accurate within the range 25-1000 ng/ml. The mean within-run and between-run coefficients of variation were < 6% at 25 ng/ml and greater concentrations. The mean accuracy of quality control standards was generally within +/- 5% of the nominal concentration. Recovery of I and internal standard from plasma was approximately 100% over the entire assay range irrespective of species.
已开发出一种使用紫外分光光度检测的反相高效液相色谱（HPLC）分析法，用于测定大鼠、犬和人血浆中3-丁酰基-4-（2-甲基苯基氨基）-8-（2-羟基乙氧基）喹啉（I）的浓度。分析前，血浆样品中的蛋白质用含有3-丁酰基-4-（2-甲基苯基氨基）-8-甲氧基喹啉作为内标的乙腈沉淀。上清液无需进一步净化即可注入HPLC柱。该分析需要200微升血浆，在25-1000 ng/ml范围内精确且准确。在25 ng/ml及更高浓度下，批内和批间变异系数均值<6%。质量控制标准品的平均准确度通常在标称浓度的+/-5%范围内。无论何种物种，I和内标在整个分析范围内从血浆中的回收率约为100%。
