Swagzdis J E, Mico B A
J Pharm Sci. 1986 Jan;75(1):90-1. doi: 10.1002/jps.2600750122.
A sensitive, specific, and accurate assay for 4-(2-di-N,N-propylaminoethyl)-2-(3H)-indolone, 1 (SK&F 101468), in plasma was developed using high-performance liquid chromatography with UV detection. The method involves sample preparation by solid-phase extraction, elution of 1 and the internal standard with a volatile solvent, concentration, and reversed-phase chromatography in the presence of an ion-pairing agent. Using 1 mL of plasma, 5 ng/mL of 1 is detectable and 10 ng/mL of 1 can be quantitated. The recovery of 1 and internal standard from plasma is greater than 95%. The within-day precision of this method at 17.5, 219, and 395 ng/mL is 3.4, 1.3, and 1.5%, respectively. The between-day precision at these concentrations is 6.0, 1.9, and 2.6%, with a mean accuracy of 100.6, 98.3, and 100.2%, respectively. Stability studies indicate that 1 is stable in plasma at -80 degrees C for less than or equal to 180 d.
采用带紫外检测的高效液相色谱法，开发了一种灵敏、特异且准确的检测血浆中4-(2-二-N,N-丙基氨基乙基)-2-(3H)-吲哚酮1（SK&F 101468）的方法。该方法包括通过固相萃取进行样品制备，用挥发性溶剂洗脱1和内标，浓缩，并在离子对试剂存在下进行反相色谱分析。使用1 mL血浆，可检测到5 ng/mL的1，且可定量10 ng/mL的1。1和内标从血浆中的回收率大于95%。该方法在17.5、219和395 ng/mL时的日内精密度分别为3.4%、1.3%和1.5%。在这些浓度下的日间精密度分别为6.0%、1.9%和2.6%，平均准确度分别为100.6%、98.3%和100.2%。稳定性研究表明，1在-80℃血浆中稳定时间小于或等于180天。
