Takahashi T, Cho H I, Kublin C L, Cintron C
Schepens Eye Research Institute, Harvard Medical School, Boston, Massachusetts.
J Histochem Cytochem. 1993 Oct;41(10):1447-57. doi: 10.1177/41.10.8245404.
Keratan sulfate proteoglycan (KSPG) and dermatan sulfate proteoglycan (DSPG) are associated with collagen fibrils in adult rabbit cornea. Because certain cytochemical data suggested that proteoglycans are associated with type VI collagen in the fetal rabbit cornea, we developed polyclonal antibodies specific to the core proteins of rabbit corneal KSPG (lumican and/or fibromodulin) and DSPG (decorin and/or biglycan) and used the antibodies as immunocytochemical probes to determine proteoglycan ultrastructural location. Immunogold particles were associated with Type VI collagen filaments but not with collagen fibrils in fetal and neonate rabbit cornea. Association of corneal KSPG and DSPG with Type VI collagen was immunocytochemically confirmed with monoclonal antibodies to low-sulfated keratan sulfate glycosaminoglycan (GAG) and chondroitin-4-sulfate GAG of DSPG. The monospecificity of the polyclonal and monoclonal antibodies and exclusive binding of antibodies to Type VI collagen filaments, together with previous chemical identification of low-sulfated KSPG and DSPG in developing rabbit cornea, support our contention that corneal proteoglycans in developing rabbit cornea are associated with Type VI collagen.
硫酸角质素蛋白聚糖（KSPG）和硫酸皮肤素蛋白聚糖（DSPG）与成年兔角膜中的胶原纤维相关。由于某些细胞化学数据表明蛋白聚糖与胎兔角膜中的VI型胶原相关，我们制备了针对兔角膜KSPG（核心蛋白聚糖和/或纤调蛋白）和DSPG（饰胶蛋白聚糖和/或双糖链蛋白聚糖）核心蛋白的多克隆抗体，并将这些抗体用作免疫细胞化学探针来确定蛋白聚糖的超微结构定位。在胎兔和新生兔角膜中，免疫金颗粒与VI型胶原丝相关，但与胶原纤维无关。用针对低硫酸化硫酸角质素糖胺聚糖（GAG）和DSPG的硫酸软骨素-4-硫酸酯GAG的单克隆抗体免疫细胞化学证实了角膜KSPG和DSPG与VI型胶原的相关性。多克隆和单克隆抗体的单特异性以及抗体与VI型胶原丝的特异性结合，再加上之前对发育中的兔角膜中低硫酸化KSPG和DSPG的化学鉴定，支持了我们的观点，即发育中的兔角膜中的角膜蛋白聚糖与VI型胶原相关。
