Reed M J, Puolakkainen P, Lane T F, Dickerson D, Bornstein P, Sage E H
Department of Medicine, University of Washington, Seattle.
J Histochem Cytochem. 1993 Oct;41(10):1467-77. doi: 10.1177/41.10.8245406.
SPARC and thrombospondin 1 (TSP-1) are secreted glycoproteins expressed by similar types of cells in culture and in tissues. To compare these two proteins in vivo, we analyzed the differential expression of SPARC and TSP-1 during wound repair. Full-thickness incision wounds were made in rats and biopsied at 12 hr-14 days. Antibodies against SPARC revealed an increased proportion of immunoreactive fibroblastic cells at the wound edge at 3 days with maximal numbers at 7 days. In situ hybridization for SPARC produced results consistent with those of immunohistochemistry. With combined immunohistochemistry and in situ hybridization, some of the macrophages at the wound edge expressed SPARC mRNA. In contrast, immunoreactivity for TSP-1 was extracellular; expression at the wound edge was noted at 12 hr and was maximal at 1-2 days. TSP-1 mRNA was found in the thrombus, but not at the wound edge. In conclusion, SPARC and TSP-1 have contrasting roles during wound healing. SPARC expression from the middle through late stages of repair was consistent with its previously proposed functions in remodeling; in contrast, the transient expression of TSP-1 early in repair might facilitate the action of other proteins in recruitment and/or proliferation of cells in the healing wound.
富含半胱氨酸的酸性分泌蛋白（SPARC）和血小板反应蛋白1（TSP - 1）是在培养细胞和组织中由相似类型细胞分泌的糖蛋白。为了在体内比较这两种蛋白，我们分析了伤口修复过程中SPARC和TSP - 1的差异表达。在大鼠身上制作全层切口伤口，并在12小时至14天进行活检。抗SPARC抗体显示，在3天时伤口边缘免疫反应性成纤维细胞比例增加，7天时数量最多。SPARC的原位杂交结果与免疫组织化学结果一致。通过免疫组织化学和原位杂交相结合的方法，发现伤口边缘的一些巨噬细胞表达SPARC mRNA。相比之下，TSP - 1的免疫反应性存在于细胞外；在12小时时伤口边缘出现表达，1 - 2天时达到最大值。TSP - 1 mRNA在血栓中发现，但不在伤口边缘。总之，SPARC和TSP - 1在伤口愈合过程中具有相反的作用。从修复中期到后期SPARC的表达与其先前提出的在重塑中的功能一致；相比之下，TSP - 1在修复早期的短暂表达可能有助于其他蛋白在愈合伤口中细胞募集和/或增殖方面发挥作用。
