Malvoisin E, Wild T F
Institut Pasteur de Lyon, Unité d'Immunologie et Stratégie Vaccinale, France.
J Gen Virol. 1993 Nov;74 ( Pt 11):2365-72. doi: 10.1099/0022-1317-74-11-2365.
We have investigated the structure and interaction of the measles virus (MV) glycoproteins expressed at the cell membrane. Cross-linking studies with a variety of chemicals stabilized dimeric forms of the haemagglutinin (HA) or fusion (F) proteins, although by sucrose density gradient analysis, oligomers corresponding to tetramers and larger were observed for both proteins. In cells in which both HA and F were expressed at the surface, their close association was shown by cross-linking and co-immunoprecipitation.
我们研究了在细胞膜上表达的麻疹病毒（MV）糖蛋白的结构与相互作用。使用多种化学物质进行的交联研究稳定了血凝素（HA）或融合（F）蛋白的二聚体形式，不过通过蔗糖密度梯度分析，观察到这两种蛋白都存在对应于四聚体及更大聚合体的寡聚体。在表面同时表达HA和F的细胞中，交联和共免疫沉淀显示了它们的紧密关联。
