Berencsi K, Rando R F, deTaisne C, Paoletti E, Plotkin S A, Gonczol E
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104-4268.
J Gen Virol. 1993 Nov;74 ( Pt 11):2507-12. doi: 10.1099/0022-1317-74-11-2507.
A murine model of the cytotoxic T lymphocyte (CTL) response to glycoprotein B (gB) of human cytomegalovirus (HCMV) was developed based on the use of adenovirus (Ad) and vaccinia virus (Vac) recombinants expressing gB. Mice of different major histocompatibility haplotypes [CBA (H-2k), BALB/k (H-2k) and BALB/c (H-2d)] infected with the Ad-gB recombinant developed an Ad-specific CTL response. However, only the H-2k mice developed a significant HCMV gB-specific CTL response, as indicated by the major histocompatibility complex class I-restricted lysis of Vac strain Copenhagen (VacC)-gB recombinant-infected target cells by H-2k mouse immune spleen cells. The VacC-gB recombinant elicited only a weak gB-specific CTL response in these mice, indicating that the observed gB-specific CTL response in mice is dependent on the expression vector used for immunization. The gB-specific cytotoxicity observed in H-2k mice was mediated by the CD8 lymphocyte subset.
基于使用表达糖蛋白B（gB）的腺病毒（Ad）和痘苗病毒（Vac）重组体，建立了针对人巨细胞病毒（HCMV）糖蛋白B的细胞毒性T淋巴细胞（CTL）应答的小鼠模型。用Ad-gB重组体感染的不同主要组织相容性单倍型的小鼠[CBA（H-2k）、BALB/k（H-2k）和BALB/c（H-2d）]产生了Ad特异性CTL应答。然而，只有H-2k小鼠产生了显著的HCMV gB特异性CTL应答，如H-2k小鼠免疫脾细胞对Vac株哥本哈根（VacC）-gB重组体感染的靶细胞进行的主要组织相容性复合体I类限制性裂解所示。VacC-gB重组体在这些小鼠中仅引发了微弱的gB特异性CTL应答，表明在小鼠中观察到的gB特异性CTL应答取决于用于免疫的表达载体。在H-2k小鼠中观察到的gB特异性细胞毒性由CD8淋巴细胞亚群介导。
