Endresz V, Berencsi K, Gönczöl E
Wistar Institute, Philadelphia, Pennsylvania, USA.
Acta Microbiol Immunol Hung. 1995;42(3):247-54.
We investigated the mechanism by which mice immunized with an adenovirus-herpes simplex glycoprotein B recombinant (Ad-HSV.gB) are protected from challenge with a vaccinia (Vac)-HSV.gB and the cognate virus, HSV. C57/BL mice immunized intraperitoneally with Ad-HSV.gB were protected against an intracerebrally inoculated lethal dose of a Vac-HSV.gB or HSV-1. CD8+ cytotoxic T lymphocytes but not interferon-gamma-dependent mechanisms play a major role in the clearance of both viruses from the central nervous system. These results demonstrate that the administration of two recombinant viruses carrying the same foreign insert for either immunization or challenge in a mouse protection model provides useful information about the protective nature of the inserted gene product.
我们研究了用腺病毒-单纯疱疹病毒糖蛋白B重组体（Ad-HSV.gB）免疫的小鼠免受痘苗病毒（Vac）-HSV.gB和同源病毒HSV攻击的机制。用Ad-HSV.gB腹腔内免疫的C57/BL小鼠可抵御脑内接种致死剂量的Vac-HSV.gB或HSV-1。CD8 + 细胞毒性T淋巴细胞而非干扰素-γ依赖性机制在从中枢神经系统清除这两种病毒中起主要作用。这些结果表明，在小鼠保护模型中，给予携带相同外源插入片段的两种重组病毒进行免疫或攻击，可为插入基因产物的保护性质提供有用信息。
