McLaughlin-Taylor E, Willey D E, Cantin E M, Eberle R, Moss B, Openshaw H
Department of Molecular Biochemistry, City of Hope National Medical Center, Duarte, California 91010.
J Gen Virol. 1988 Jul;69 ( Pt 7):1731-4. doi: 10.1099/0022-1317-69-7-1731.
Spleen cells from BALB/c (H-2d) mice vaccinated with vgB11, a recombinant vaccinia virus which expresses glycoprotein B (gB) of herpes simplex virus type 1 (HSV-1), lysed EMT6 (H-2d) target cells infected with vgB11 or with HSV-1 but did not lyse uninfected EMT6 cells or infected L-929 (H-2k) target cells. Unlabelled target cell competition of lysis showed that only syngeneic cells infected with vgB11 or HSV-1 inhibited lysis of radiolabelled HSV-1-infected targets. These results demonstrate that vgB11 induces H-2-restricted anti-HSV-1 cytotoxic T lymphocytes and that gB is the target antigen.
用vgB11（一种表达1型单纯疱疹病毒（HSV-1）糖蛋白B（gB）的重组痘苗病毒）接种的BALB/c（H-2d）小鼠的脾细胞，可裂解被vgB11或HSV-1感染的EMT6（H-2d）靶细胞，但不能裂解未感染的EMT6细胞或被感染的L-929（H-2k）靶细胞。未标记靶细胞的裂解竞争实验表明，只有被vgB11或HSV-1感染的同基因细胞能抑制放射性标记的HSV-1感染靶细胞的裂解。这些结果表明，vgB11可诱导H-2限制性抗HSV-1细胞毒性T淋巴细胞，且gB是靶抗原。
