Burczynski F J, Moran J B, Cai Z S
Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
J Pharmacol Exp Ther. 1993 Nov;267(2):714-9.
Experiments were conducted comparing palmitate clearance by hepatocyte monolayers, polyethylene and that predicted by the diffusion-reaction model. The objective was to learn whether the albumin-dependent enhancement of unbound palmitate clearance was wholly attributable to codiffusion of bound and unbound fatty acid across an unstirred fluid layer, or whether the uptake flux was also contributed by facilitated dissociation of albumin-fatty acid complexes at the cell surface. We use a model-independent formulation of the facilitation phenomenon to explore this question at low concentrations of albumin. The central finding that facilitation occurs takes into consideration albumin-dependent changes in solution viscosity, endogenous albumin secretion and the adsorption of albumin to the polyethylene surface.
进行了实验，比较了肝细胞单层、聚乙烯对棕榈酸酯的清除率以及扩散反应模型预测的清除率。目的是了解未结合棕榈酸酯清除率的白蛋白依赖性增强是否完全归因于结合和未结合脂肪酸在未搅拌液层中的共扩散，或者摄取通量是否也由细胞表面白蛋白 - 脂肪酸复合物的易化解离所贡献。我们使用促进现象的模型无关公式，在低浓度白蛋白条件下探讨这个问题。促进作用发生这一核心发现考虑了溶液粘度的白蛋白依赖性变化、内源性白蛋白分泌以及白蛋白在聚乙烯表面的吸附。
