Burczynski F J, Moran J B, Cai Z S
Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Can J Physiol Pharmacol. 1993 Dec;71(12):863-7. doi: 10.1139/y93-130.
We used the clearance ratio test to determine whether the presence of albumin enhances the uptake of palmitate by hepatocyte monolayers. If uptake is facilitated, then the ratio of palmitate clearance by hepatocytes in the presence of 600 and 300 microM albumin (i.e., Clt600/Clt300) will exceed the square root of the ratio of the unbound fractions at 600 and 300 microM albumin (i.e., square root of alpha 600/alpha 300). The latter value was determined using the heptane-water partitioning technique. The hepatocyte clearance ratio (0.92 +/- 0.06) exceeded the predicted value (0.80) by 15% (p < 0.05). The observed clearance rate also exceeded that predicted by the diffusion-reaction model.
我们采用清除率试验来确定白蛋白的存在是否会增强单层肝细胞对棕榈酸酯的摄取。如果摄取得到促进，那么在存在600和300微摩尔白蛋白的情况下，肝细胞对棕榈酸酯的清除率之比（即Clt600/Clt300）将超过600和300微摩尔白蛋白时未结合部分之比的平方根（即α600/α300的平方根）。后一个值是使用庚烷 - 水分配技术测定的。肝细胞清除率（0.92±0.06）比预测值（0.80）高出15%（p<0.05）。观察到的清除率也超过了扩散反应模型预测的值。
