Ohkuni H, Todome Y, Mizuse M, Ohtani N, Suzuki H, Igarashi H, Hashimoto Y, Ezaki T, Harada K, Imada Y
Division of Immunology, Nippon Medical School, Kanagawa, Japan.
J Med Microbiol. 1993 Nov;39(5):352-62. doi: 10.1099/00222615-39-5-352.
A bacteriological study of isolates from the oral cavity of patients with Kawasaki disease (KD), age-matched non-KD patients and healthy children, showed that over half the KD and control isolates had gram-positive, catalase-negative cocci. About 50% of these organisms were identified as viridans streptococci by means of an API Strep 20 kit. Further identification by fluorometric DNA-DNA hybridisation demonstrated that the predominant species were S. oralis and S. mitis, each of which accounted for 25% of the isolates of viridans streptococci; 40% of viridans strains were unidentifiable; and S. sanguis and S. parasanguis were minor components. Studies in vivo showed that insertion of culture supernates of most of the viridans streptococci increased capillary permeability and induced redness with swelling and occasional bleeding in rabbit skin. One-third of S. mitis strains and one-fifth of the unidentified strains caused aggregation of human blood platelets, whereas S. oralis and other strains had no such effect. The distribution of extracellular lipoteichoic acids and glucan produced in the presence of sucrose was also examined. There were no significant differences in the recovery rate of viridans streptococci forming these biologically active extracellular products between KD and control groups.
一项针对川崎病（KD）患者、年龄匹配的非KD患者以及健康儿童口腔分离株的细菌学研究表明，超过半数的KD和对照分离株含有革兰氏阳性、过氧化氢酶阴性球菌。通过API Strep 20试剂盒，约50%的此类微生物被鉴定为草绿色链球菌。通过荧光DNA-DNA杂交进一步鉴定显示，主要菌种为口腔链球菌和缓症链球菌，每种菌在草绿色链球菌分离株中均占25%；40%的草绿色链球菌菌株无法鉴定；血链球菌和副血链球菌为次要成分。体内研究表明，大多数草绿色链球菌培养上清液的注入会增加兔皮肤的毛细血管通透性，并导致发红、肿胀以及偶尔出血。三分之一的缓症链球菌菌株和五分之一的未鉴定菌株会引起人血小板聚集，而口腔链球菌和其他菌株则无此作用。还检测了在蔗糖存在下产生的细胞外脂磷壁酸和葡聚糖的分布。在形成这些具有生物活性的细胞外产物的草绿色链球菌回收率方面，KD组和对照组之间没有显著差异。
