Bach A, Böhrer H, Motsch J, Martin E, Geiss H K, Sonntag H G
Department of Anesthesiology, University of Heidelberg, Germany.
J Surg Res. 1993 Dec;55(6):640-6. doi: 10.1006/jsre.1993.1197.
A novel catheter pretreated with the antiseptics chlorhexidine and silver-sulfadiazine, designed to reduce catheter-related colonization and infection, was tested in both in vitro and in vivo studies. In vitro experiments demonstrated the long-lasting antibacterial properties of this catheter. For the in vivo study a total of 40 rats divided into different experimental groups were used. Colonization rates of both antiseptic bonded (AS) and control (C) catheters were assessed either three (-3) or seven (-7) days after implantation and local challenge using live Staphylococcus epidermidis ATCC 35984 with 10(7) colony-forming units (cfu) per inoculum. At the time of removal, catheters, organ specimens, and blood samples were taken for cultivation. Significant reductions in the magnitude of colonization of the antiseptic catheters by the test organism were observed in all groups. The average number of cfu colonizing control segments exceeded those found on the treated catheter segments by log 3 (C3/AS3) and log 5 (C7/AS7), respectively (C3: 1.2 x 10(6) +/- 4.1 x 10(5) cfu/segment and AS3: 1.8 x 10(3) +/- 6.6 x 10(2); C7: 2.7 x 10(5) +/- 8.6 x 10(4) and AS7: 1.1 +/- 0.7; mean +/- standard error of the mean, all differences between matching groups statistically significant, Wilcoxon rank sum test, P < 0.0001). These data suggest that antiseptic catheters may substantially decrease the magnitude of catheter-related microbial colonization and subsequent catheter-related infections and may offer a more effective alternative to current methods.
一种新型导管，经抗菌剂洗必泰和磺胺嘧啶银预处理，旨在减少与导管相关的定植和感染，已在体外和体内研究中进行了测试。体外实验证明了这种导管具有持久的抗菌性能。在体内研究中，共使用了40只大鼠并将其分为不同的实验组。在植入并使用每接种物含10(7)个菌落形成单位(cfu)的活表皮葡萄球菌ATCC 35984进行局部攻击后3天(-3)或7天(-7)，评估抗菌剂结合(AS)导管和对照(C)导管的定植率。在移除导管时，采集导管、器官标本和血样进行培养。在所有组中均观察到测试微生物在抗菌导管上的定植量显著减少。定植于对照段的cfu平均数量分别比处理过的导管段多log 3(C3/AS3)和log 5(C7/AS7)(C3: 1.2 x 10(6)+/- 4.1 x 10(5) cfu/段，AS3: 1.8 x 10(3)+/- 6.6 x 10(2); C7: 2.7 x 10(5)+/- 8.6 x 10(4)，AS7: 1.1 +/- 0.7;平均值+/-平均值的标准误差，匹配组之间的所有差异均具有统计学意义，Wilcoxon秩和检验，P < 0.0001)。这些数据表明，抗菌导管可能会大幅降低与导管相关的微生物定植量以及随后与导管相关的感染，并可能为当前方法提供更有效的替代方案。
