蜡样芽孢杆菌菌株产生的肠毒素的部分分离及某些特性
Partial isolation and some properties of enterotoxin produced by Bacillus cereus strains.
作者信息
Guaycurus T V, Vicente A C, de Simone S G, Rabinovitch L
机构信息
Departamento de Bacteriologia, Instituto Oswaldo Cruz, Rio de Janeior, Brasil.
出版信息
Mem Inst Oswaldo Cruz. 1993 Jan-Mar;88(1):131-4. doi: 10.1590/s0074-02761993000100020.
Extracellular proteins produced by Bacillus cereus AL-42 and AL-15 were fractioned by chromatography on QAE-Sephadex and Sephadex G75. This last chromatographic process resulted in three peaks. The major peak showed vascular permeability activity to rabbits, lethality to mice, and cytotoxicity to Vero and Hela cells. The analysis by SDS-PAGE after ultrafiltration confirm recent findings that the enterotoxin is a compound with molecular mass > 30,000.
蜡样芽孢杆菌AL - 42和AL - 15产生的细胞外蛋白质通过QAE - 葡聚糖凝胶和葡聚糖凝胶G75柱色谱进行分离。最后的色谱过程产生了三个峰。主要峰对兔子表现出血管通透性活性，对小鼠具有致死性，对Vero和Hela细胞具有细胞毒性。超滤后通过SDS - PAGE分析证实了最近的发现，即该肠毒素是一种分子量大于30,000的化合物。
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