Bashey R I, Sampson P M, Jimenez S A, Heimer R
Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA.
Matrix. 1993 Sep;13(5):363-71. doi: 10.1016/s0934-8832(11)80041-7.
Lyophilized mid-wall left ventricular myocardial tissues of the long-tailed non-human primate, Macaca fascicularis, were examined for the presence of glycosaminoglycans and chondroitin/dermatan sulfate proteoglycans. Mean uronic acid concentration in all samples was 0.97 +/- 0.27 micrograms per mg dry weight myocardium. The distribution of the glycosaminoglycans in the myocardium, determined by cellulose acetate strip electrophoresis was 62 +/- 4% heparan sulfate, 20 +/- 6% hyaluronan, and 16 +/- 5% chondroitin/dermatan sulfate. The analysis of chondroitin/dermatan sulfate proteoglycans, done directly on the extracts of lyophilized myocardium using agarose-acrylamide gel electrophoresis and Western blotting with monoclonal antibodies to various carbohydrate epitopes and with polyclonal antibodies to the protein core, showed the presence of biglycan and decorin. That these two and no other chondroitin/dermatan sulfates were present was established by core protein analysis using SDS PAGE and Western blotting. Quantification of chondroitin/dermatan sulfate proteoglycans uncovered high individual specific variability of the chondroitin/dermatan sulfate epitopes, but only moderate variability of biglycan and decorin core proteins. The variability of the chondroitin/dermatan sulfate epitopes is most likely related to individual specific differences in chain number, iduronate content and sulfation patterns of biglycan and decorin.
对食蟹猴这种长尾非人灵长类动物冻干的左心室中层心肌组织进行检测，以确定其中是否存在糖胺聚糖以及硫酸软骨素/硫酸皮肤素蛋白聚糖。所有样本中糖醛酸的平均浓度为每毫克干重心肌0.97±0.27微克。通过醋酸纤维素条电泳测定，心肌中糖胺聚糖的分布为：62±4%为硫酸乙酰肝素，20±6%为透明质酸，16±5%为硫酸软骨素/硫酸皮肤素。直接对冻干心肌提取物进行硫酸软骨素/硫酸皮肤素蛋白聚糖分析，采用琼脂糖-丙烯酰胺凝胶电泳以及用针对各种碳水化合物表位的单克隆抗体和针对蛋白核心的多克隆抗体进行蛋白质印迹法，结果显示存在双糖链蛋白聚糖和核心蛋白聚糖。通过使用SDS-PAGE和蛋白质印迹法进行核心蛋白分析，确定存在这两种而非其他硫酸软骨素/硫酸皮肤素。硫酸软骨素/硫酸皮肤素蛋白聚糖的定量分析发现，硫酸软骨素/硫酸皮肤素表位存在高度的个体特异性变异，但双糖链蛋白聚糖和核心蛋白聚糖的核心蛋白仅存在中度变异。硫酸软骨素/硫酸皮肤素表位的变异很可能与双糖链蛋白聚糖和核心蛋白聚糖在链数、艾杜糖醛酸含量和硫酸化模式方面的个体特异性差异有关。
