Ohishi H, Binette J P, Schmid K
Clin Chim Acta. 1986 Apr 30;156(2):157-63. doi: 10.1016/0009-8981(86)90149-x.
The glycosaminoglycans of the myocardium of an individual who died of acute carbon monoxide poisoning were quantified by two-dimensional electrophoresis on cellulose acetate membranes. The total glycosaminoglycan content was found to be approximately twice the normal value. In contrast to the content of each glycosaminoglycan of the normal heart, the level of the chondroitin sulfates of this patient's myocardium was found to be markedly increased whereas that of hyaluronic acid was decreased and dermatan sulfate could not be detected. Further, this tissue contained significant amounts of keratan sulfate and an oversulfated dermatan sulfate, glycosaminoglycans not found in normal myocardium. Of particular interest was the presence of two unusual oversulfated chondroitin sulfates (chondroitin sulfates D and E).
通过在醋酸纤维素膜上进行二维电泳，对死于急性一氧化碳中毒个体的心肌糖胺聚糖进行了定量分析。发现总糖胺聚糖含量约为正常值的两倍。与正常心脏中每种糖胺聚糖的含量相比，该患者心肌中硫酸软骨素的水平显著升高，而透明质酸水平降低，且未检测到硫酸皮肤素。此外，该组织含有大量硫酸角质素和一种过度硫酸化的硫酸皮肤素，这些糖胺聚糖在正常心肌中未发现。特别值得关注的是存在两种异常的过度硫酸化硫酸软骨素（硫酸软骨素D和E）。
