齿垢密螺旋体ATCC 33520表面定位蛋白和碳水化合物部分在其与培养的大鼠腭上皮细胞黏附中的作用。

Involvement of treponemal surface-located protein and carbohydrate moieties in the attachment of Treponema denticola ATCC 33520 to cultured rat palatal epithelial cells.

作者信息

Keulers R A, Maltha J C, Mikx F H, Wolters-Lutgerhorst J M

机构信息

Laboratory of Oral Histology, University of Nijmegen, The Netherlands.

出版信息

Oral Microbiol Immunol. 1993 Aug;8(4):236-41. doi: 10.1111/j.1399-302x.1993.tb00566.x.

DOI:10.1111/j.1399-302x.1993.tb00566.x

PMID:8247611

Abstract

We studied the nature of attachment of Treponema denticola ATCC 33520 to a microscopically distinct population of rounded rat palatal epithelial cells. The motility of the freshly harvested spirochetes appeared not be a prerequisite for attachment. Treatment of T. denticola ATCC 3350 with proteinase-K, heat, glutaraldehyde, formaldehyde and periodate oxidation decreased the attachment to the rounded rat palatal epithelial cells, indicating the involvement of protein and carbohydrate moieties. Trypsin treatment had no effect on the attachment. The attachment of T. denticola ATCC 33520 was decreased after treatment with native non-immune rabbit serum, native polyclonal rabbit serum, D-mannose, N-acetyl-D-galactosamine and sialic acid. The results indicate that the attachment of T. denticola ATCC 33520 to rounded rat palatal epithelial cells is mediated by trypsin-resistant adhesin(s) of protein and carbohydrate nature, with affinity for D-mannose, N-acetyl-D-galactosamine and sialic acid.

摘要

我们研究了具核梭杆菌ATCC 33520与一群在显微镜下可区分的圆形大鼠腭上皮细胞的附着性质。新鲜收获的螺旋体的运动性似乎不是附着的先决条件。用蛋白酶K、加热、戊二醛、甲醛和高碘酸盐氧化处理具核梭杆菌ATCC 3350会降低其与圆形大鼠腭上皮细胞的附着，表明蛋白质和碳水化合物部分参与其中。胰蛋白酶处理对附着没有影响。用天然非免疫兔血清、天然多克隆兔血清、D-甘露糖、N-乙酰-D-半乳糖胺和唾液酸处理后，具核梭杆菌ATCC 33520的附着减少。结果表明，具核梭杆菌ATCC 33520与圆形大鼠腭上皮细胞的附着是由具有蛋白质和碳水化合物性质、对D-甘露糖、N-乙酰-D-半乳糖胺和唾液酸有亲和力的抗胰蛋白酶粘附素介导的。