齿垢密螺旋体菌株与不同来源上皮细胞单层的附着。
Attachment of Treponema denticola strains to monolayers of epithelial cells of different origin.
作者信息
Keulers R A, Maltha J C, Mikx F H, Wolters-Lutgerhorst J M
机构信息
Laboratory of Oral Histology, University of Nijmegen, The Netherlands.
出版信息
Oral Microbiol Immunol. 1993 Apr;8(2):84-8. doi: 10.1111/j.1399-302x.1993.tb00550.x.
The attachment of 10 different Treponema denticola strains to monolayers of 4 types of epithelial cells derived from rat palatal epithelium, guinea pig ear, human buccal epithelium and human corneal epithelium was screened microscopically. Most T. denticola strains were able to attach to all four types of epithelial cells. The T. denticola strains seemed to attach better to epithelial cells derived from primary cultured material. The T. denticola strains showed different degrees of attachment. Scanning electron microscopy studies revealed that the attachment of T. denticola was not only tip-associated but occurred also at random points in close contact with microvilli of the epithelial cells. Attached spirochetes were non-uniformly distributed over the monolayers, indicating the presence of receptive subpopulations of epithelial cells in the monolayers.
通过显微镜筛选了10种不同的齿垢密螺旋体菌株对源自大鼠腭上皮、豚鼠耳、人颊上皮和人角膜上皮的4种上皮细胞单层的附着情况。大多数齿垢密螺旋体菌株能够附着于所有4种上皮细胞类型。齿垢密螺旋体菌株似乎对源自原代培养材料的上皮细胞附着得更好。齿垢密螺旋体菌株表现出不同程度的附着。扫描电子显微镜研究显示，齿垢密螺旋体的附着不仅与菌尖相关，也发生在与上皮细胞微绒毛紧密接触的随机点处。附着的螺旋体在单层细胞上分布不均匀，表明单层上皮细胞中存在易接受的亚群。
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