Haapasalo M, Hannam P, McBride B C, Uitto V J
Department of Microbiology, University of Columbia, Vancouver, Canada.
Oral Microbiol Immunol. 1996 Jun;11(3):156-60. doi: 10.1111/j.1399-302x.1996.tb00351.x.
Binding of Treponema denticola ATCC 35405 to glycosaminoglycans, fibrinogen, type I collagen and porcine periodontal ligament epithelial cells was studied using an enzyme-linked immunosorbent assay. T. denticola bound to hyaluronan (hyaluronic acid) and its hexameric fragments. whereas little or no binding was detected to chondroitin-4-sulfate or dermatan sulfate proteoglycan. Binding of T. denticola to hyaluronan gradually increased during the 2-h incubation time. In contrast, binding to fibrinogen and type I collagen was more rapid, peaking within 5 min. T. denticola also bound to microbeads coated with hyaluronan and formed visible aggregates in solution. Pretreatment of the bacteria with hyaluronan or fibrinogen inhibited binding to hyaluronan. Gelatin, bovine serum albumin, chondroitin-4-sulfate, chondroitin-6-sulfate, heparin, dermatan sulfate, glucuronic acid, N-acetylglucosamine and N-acetyl-galactosamine did not inhibit binding. Binding was also inhibited by heating T. denticola and by pretreatment of the spirochetes with sodium periodate, phenylmethylsulfonyl fluoride, and p-chloromercurybenzoic acid. All these treatments also inhibited the chymotrypsin-like activity of T. denticola. Hyaluronan strongly inhibited binding of T. denticola to epithelial cells, whereas the other glycosaminoglycans and N-acetyl-glucosamine did not. The results show that T. denticola binds to hyaluronan, possibly by a mechanism involving the chymotrypsin-like surface protein of T. denticola.
利用酶联免疫吸附测定法研究了具核梭杆菌ATCC 35405与糖胺聚糖、纤维蛋白原、I型胶原和猪牙周膜上皮细胞的结合情况。具核梭杆菌与透明质酸及其六聚体片段结合，而与硫酸软骨素-4或硫酸皮肤素蛋白聚糖的结合很少或未检测到。在2小时的孵育时间内，具核梭杆菌与透明质酸的结合逐渐增加。相比之下，与纤维蛋白原和I型胶原的结合更快，在5分钟内达到峰值。具核梭杆菌也与包被有透明质酸的微珠结合，并在溶液中形成可见的聚集体。用透明质酸或纤维蛋白原预处理细菌可抑制其与透明质酸的结合。明胶、牛血清白蛋白、硫酸软骨素-4、硫酸软骨素-6、肝素、硫酸皮肤素、葡萄糖醛酸、N-乙酰葡糖胺和N-乙酰半乳糖胺均不抑制结合。加热具核梭杆菌以及用过碘酸钠、苯甲基磺酰氟和对氯汞苯甲酸预处理螺旋体也可抑制结合。所有这些处理也抑制了具核梭杆菌的类胰凝乳蛋白酶活性。透明质酸强烈抑制具核梭杆菌与上皮细胞的结合，而其他糖胺聚糖和N-乙酰葡糖胺则无此作用。结果表明，具核梭杆菌可能通过一种涉及具核梭杆菌类胰凝乳蛋白酶样表面蛋白的机制与透明质酸结合。
