Cloning of a gene required for the alkaliphily of alkaliphilic Bacillus sp. strain C-125.
作者信息
Aono R, Hayakawa A, Hashimoto M, Kaneko H, Nakamura S, Horikoshi K
机构信息
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
出版信息
Nucleic Acids Symp Ser. 1993(29):139-40.
PMID:8247741
Abstract
An alkali-sensitive mutant was obtained from alkaliphilic Bacillus sp. strain C-125. A DNA fragment required for the alkaliphily was cloned from the chromosomal DNA of strain C-125 by using the alkali-sensitive mutant as a host. The nucleotide sequence of the cloned DNA fragment indicated the occurrence of several ORFs. Comparison of these ORFs with sequences in the available databases revealed no closely related sequences.
摘要
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