Andrus A, Theisen P D, McCollum C
Division of Perkin-Elmer Co., Foster City, CA 94404.
Nucleic Acids Symp Ser. 1993(29):5-6.
A series of dialkylformamidine protected deoxyadenosine phosphoramidites were prepared for automated, solid-support DNA synthesis. The set of Abz, Gdmf, Cbz, T phosphoramidites gave high purity and high yield oligonucleotides, with rapid and complete deprotection at 65 degrees C in one hour. Different times and temperatures of exposure to concentrated ammonium hydroxide were examined to establish the optimum conditions for deprotection of oligonucleotides.
制备了一系列二烷基甲脒保护的脱氧腺苷亚磷酰胺，用于自动化的固相DNA合成。Abz、Gdmf、Cbz、T亚磷酰胺组合产生了高纯度和高产率的寡核苷酸，在65℃下1小时内可快速完全脱保护。研究了暴露于浓氢氧化铵的不同时间和温度，以确定寡核苷酸脱保护的最佳条件。
