Matray T J, Yoo D J, McMinn D L, Greenberg M M
Department of Chemistry, Colorado State University, Fort Collins 80523, USA.
Bioconjug Chem. 1997 Mar-Apr;8(2):99-102. doi: 10.1021/bc970013a.
Oligonucleotides containing a 3'-alkylcarboxylic acid are isolated using a Pd(0)-catalyzed cleavage reaction, in yields that are in most cases within experimental error of those isolated using standard oligonucleotide cleavage conditions (concentrated NH4OH). In contrast to results obtained with photolabile solid phase synthesis supports, no reduction in isolated yields of the oligonucleotides is observed when their length is increased from 20 to 40 nucleotides. The oligonucleotides are characterized by anion exchange HPLC, electrospray mass spectrometry, and enzymatic digestion. When methyl phosphoramidites are employed in the synthesis of the biopolymers, 3 serves as an orthogonal solid phase oligonucleotide synthesis support.
含有3'-烷基羧酸的寡核苷酸通过钯(0)催化的裂解反应进行分离，在大多数情况下，其产率与使用标准寡核苷酸裂解条件（浓氨水）分离得到的产率在实验误差范围内。与使用光不稳定固相合成载体获得的结果相反，当寡核苷酸长度从20个核苷酸增加到40个核苷酸时，未观察到其分离产率降低。这些寡核苷酸通过阴离子交换高效液相色谱、电喷雾质谱和酶切进行表征。当在生物聚合物的合成中使用甲基亚磷酰胺时，3用作正交固相寡核苷酸合成载体。
