核酸的亲和凝胶电泳。含孔雀石绿的水溶性聚合物与双链DNA之间的相互作用。
Affinity gel electrophoresis of nucleic acids. The interaction between water soluble polymers having malachite green and double-stranded DNAs.
作者信息
Inami Y, Nakamura K, Ishiba H, Kishida A, Akashi M
机构信息
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, Japan.
出版信息
Nucleic Acids Symp Ser. 1993(29):77-8.
Copolymers prepared by the free radical copolymerization of acrylamide and vinyl malachite green were immobilized in an agarose gel matrix to produce a novel affinity gel for double-stranded DNAs. Relative electrophoretic mobilities of calf thymus DNA and poly(dA-dT)2 strongly depended on the amount of vinyl malachite green to show the strong interaction between DNAs and the copolymers. But any affinity interaction was not observed between poly(dG-dC)2 and the copolymers.
通过丙烯酰胺与乙烯基孔雀石绿的自由基共聚反应制备的共聚物被固定在琼脂糖凝胶基质中，以制备一种新型的双链DNA亲和凝胶。小牛胸腺DNA和聚（dA-dT）2的相对电泳迁移率强烈依赖于乙烯基孔雀石绿的量，表明DNA与共聚物之间存在强相互作用。但在聚（dG-dC）2与共聚物之间未观察到任何亲和相互作用。
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