Guadagno T M, Ohtsubo M, Roberts J M, Assoian R K
Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032.
Science. 1993 Dec 3;262(5139):1572-5. doi: 10.1126/science.8248807.
Cell adhesion has an essential role in regulating proliferation during the G1 phase of the cell cycle, and loss of this adhesion requirement is a classic feature of oncogenic transformation. The appearance of cyclin A messenger RNA and protein in late G1 was dependent on cell adhesion in both NRK and NIH 3T3 fibroblasts. In contrast, the expression of Cdc2, Cdk2, cyclin D1, and cyclin E was independent of adhesion in both cell lines. Transfection of NRK cells with a cyclin A complementary DNA resulted in adhesion-independent accumulation of cyclin A protein and cyclin A-associated kinase activity. These transfected cells also entered S phase and complete multiple rounds of cell division in the absence of cell adhesion. Thus, cyclin A is a target of the adhesion-dependent signals that control cell proliferation.
细胞黏附在细胞周期G1期调控细胞增殖过程中发挥着重要作用，而这种黏附需求的丧失是致癌转化的一个典型特征。在NRK和NIH 3T3成纤维细胞中，细胞周期蛋白A信使核糖核酸和蛋白质在G1晚期的出现均依赖于细胞黏附。相比之下，在这两种细胞系中，Cdc2、Cdk2、细胞周期蛋白D1和细胞周期蛋白E的表达均不依赖于黏附。用细胞周期蛋白A互补脱氧核糖核酸转染NRK细胞，导致细胞周期蛋白A蛋白和细胞周期蛋白A相关激酶活性的黏附非依赖性积累。这些转染细胞在没有细胞黏附的情况下也进入S期并完成多轮细胞分裂。因此，细胞周期蛋白A是控制细胞增殖的黏附依赖性信号的一个靶点。
