Department of Digestive Disease, Shandong Provincial Hospital Affiliated to Shandong University, No. 324, the Five Weft Seven Road, Ji'nan City, Shandong Province, 250021, People's Republic of China.
BMC Complement Med Ther. 2020 Jan 23;20(1):21. doi: 10.1186/s12906-019-2810-3.
Germacrone is one of the natural bioactive compounds found in Rhizoma curcuma essential oils. In this study, the potential anti-cancer effect of germacrone in gastric cancer cell line BGC823 was investigated.
The cell viability and proliferative activity were assessed, and cell cycle analysis was also performed. Hoechst 33258 and Annexin V/PI double staining was used for detection of cell apoptosis. Protein profiles of cell cycle-related and apoptosis-related proteins were assessed.
MTT assay revealed that germacrone had marked cytotoxicity on BGC823 cells. Germacrone induced cell cycle arrest in the G2/M phase via remarkably decreased expression levels of cyclin B1, cdc 2 and cdc 25c. In addition, the treatment with germacrone induced caspase-3 activity and PARP cleavage. These findings demonstrated the effects of germacrone on inhibiting cell proliferation through induction of G2/M phase cell cycle arrest and promotion of cell apoptosis. It also indicated that germacrone functioned through modulations of cell cycle-associated protein expression and mitochondria-mediated apoptosis.
These findings will be valuable as the molecular basis for the germacrone-mediated anti-cancer effect against gastric cancer.
姜烯酮是姜黄根茎精油中的一种天然生物活性化合物。本研究旨在探讨姜烯酮对胃癌 BGC823 细胞系的潜在抗癌作用。
评估细胞活力和增殖活性，并进行细胞周期分析。采用 Hoechst 33258 和 Annexin V/PI 双重染色法检测细胞凋亡。评估与细胞周期和细胞凋亡相关的蛋白谱。
MTT 试验显示姜烯酮对 BGC823 细胞具有显著的细胞毒性。姜烯酮通过显著降低 cyclin B1、cdc2 和 cdc25c 的表达水平，诱导细胞周期停滞在 G2/M 期。此外，姜烯酮处理诱导 caspase-3 活性和 PARP 裂解。这些发现表明姜烯酮通过诱导 G2/M 期细胞周期阻滞和促进细胞凋亡来抑制细胞增殖。这也表明姜烯酮通过调节细胞周期相关蛋白表达和线粒体介导的细胞凋亡发挥作用。
这些发现将为姜烯酮介导的抗胃癌作用提供有价值的分子基础。
