Samouris G, Nathanael B, Tsoukali-Papadopoulou H, Papadimitriou N
Laboratory of Forensic Medicine and Toxicology, Medical Faculty, Aristotelian University of Thessaloniki, Greece.
Vet Hum Toxicol. 1993 Oct;35(5):406-9.
A rapid and sensitive HPLC method for the determination of chloramphenicol (CAP) residues in albumin and yolk of eggs is described. Conditions of isolation and identification are presented in detail. The detection limit is 10 ppb and the linearity is very good up to 2500 ppb CAP. The recovery of the method is 82 and 77%, and the precision is 6.6 and 11.1% for the albumin and yolk determinations respectively. The method was tested to quantitate CAP residues in eggs of 5 laying hens to which CAP had been administered in feed at a concentration of 800 mg/kg. CAP was detected in albumin and yolk of eggs laid 3 and 8 days after the CAP administration, respectively.
描述了一种快速灵敏的高效液相色谱法，用于测定鸡蛋白蛋白和蛋黄中的氯霉素（CAP）残留量。详细介绍了分离和鉴定条件。检测限为10 ppb，在高达2500 ppb的CAP范围内线性非常好。该方法对白蛋白和蛋黄测定的回收率分别为82%和77%，精密度分别为6.6%和11.1%。对5只产蛋母鸡进行了测试，这些母鸡在饲料中以800 mg/kg的浓度投喂了CAP，分别在投喂CAP后3天和8天所产鸡蛋的白蛋白和蛋黄中检测到了CAP。
