Bories G S, Peleran J C, Wal J M
J Assoc Off Anal Chem. 1983 Nov;66(6):1521-6.
A rapid and sensitive liquid chromatographic (LC) method for determination of trace amounts of chloramphenicol (CP) in animal tissues has been developed. Chloramphenicol identity is confirmed by gas chromatography-mass spectrometry (GC-MS). CP can be routinely quantitated by LC at 10 ppb with 75% recovery, and GC-MS selected ion monitoring permits complete identification at concentrations as low as 5 ppb. Residues were determined in chicken tissues after intramuscular injection of CP. Depletion was rapid with no residues detected in muscle after 8 h, while 15-30 ppb CP was still determined and identified in the liver.
已开发出一种快速灵敏的液相色谱（LC）方法，用于测定动物组织中的痕量氯霉素（CP）。氯霉素的身份通过气相色谱-质谱联用（GC-MS）得以确认。CP可通过LC常规定量，在10 ppb时回收率为75%，而GC-MS选择离子监测能够在低至5 ppb的浓度下实现完全鉴定。在肌肉注射CP后，对鸡组织中的残留进行了测定。清除速度很快，8小时后在肌肉中未检测到残留，而在肝脏中仍可测定并鉴定出15 - 30 ppb的CP。
