Reams G, Villarreal D, Wu Z, Bauer J H
Department of Medicine, University of Missouri, Columbia 65212.
Am J Kidney Dis. 1993 Dec;22(6):851-7. doi: 10.1016/s0272-6386(12)70345-1.
A procedure for the collection, processing, extraction, separation, and measurement of renal tissue angiotensin II [ANG-(1-8)] is described. The arterial plasma and renal tissue concentrations of ANG-(1-8) were examined in anesthetized Sprague-Dawley male rats (10 to 12 weeks of age) given an intravenous saline infusion (group 1; n = 6), an intravenous bolus infusion of 0.5 microgram angiotensin I (group 2; n = 6), or an intravenous bolus of an angiotensin-converting enzyme inhibitor (group 3; n = 6). Plasma and renal tissue were collected at the peak mean arterial pressure (MAP) response. The mean (+/- SEM) concentration of ANG-(1-8) in the group given the saline vehicle was 12 +/- 2 fmol/mL in the plasma and 2.4 +/- 0.3 pmol/g in the renal tissue. The angiotensin I bolus significantly increased MAP by 40%. Following the angiotensin I infusion, at the time of peak MAP response, the concentration of the circulating and renal tissue ANG-(1-8) were 12-fold and twofold higher, respectively, compared with the saline vehicle. The angiotensin-converting enzyme inhibitor significantly decreased MAP by 10% and decreased the circulating and renal tissue ANG-(1-8) levels by 75% and 62.5%, respectively, compared with the saline vehicle. The rapid conversion of exogenous angiotensin I to ANG-(1-8) in renal tissue provides direct evidence that renal tissue can generate de novo ANG-(1-8) from a circulating precursor. Furthermore, plasma and renal tissue respond similarly to acute angiotensin-converting enzyme inhibition.
本文描述了一种用于收集、处理、提取、分离和测量肾组织血管紧张素II [ANG-(1-8)] 的方法。在静脉输注生理盐水的麻醉Sprague-Dawley雄性大鼠（10至12周龄）（第1组；n = 6）、静脉推注0.5微克血管紧张素I的大鼠（第2组；n = 6）或静脉推注血管紧张素转换酶抑制剂的大鼠（第3组；n = 6）中，检测了ANG-(1-8) 的动脉血浆和肾组织浓度。在平均动脉压（MAP）反应峰值时收集血浆和肾组织。给予生理盐水载体的组中，血浆中ANG-(1-8) 的平均（±SEM）浓度为12±2 fmol/mL，肾组织中为2.4±0.3 pmol/g。血管紧张素I推注使MAP显著升高40%。在输注血管紧张素I后，在MAP反应峰值时，循环和肾组织中ANG-(1-8) 的浓度分别比生理盐水载体组高12倍和2倍。与生理盐水载体组相比，血管紧张素转换酶抑制剂使MAP显著降低10%，循环和肾组织中ANG-(1-8) 水平分别降低75%和62.5%。肾组织中外源性血管紧张素I快速转化为ANG-(1-8) 提供了直接证据，表明肾组织可以从循环前体中从头生成ANG-(1-8)。此外，血浆和肾组织对急性血管紧张素转换酶抑制的反应相似。
