通过温度对重组大肠杆菌连续培养中cI857控制的基因表达进行精细调控。
Fine regulation of cI857-controlled gene expression in continuous culture of recombinant Escherichia coli by temperature.
作者信息
Villaverde A, Benito A, Viaplana E, Cubarsi R
机构信息
Institut de Biologia Fonamental, Universitat Autònoma de Barcelona, Bellaterra, Spain.
出版信息
Appl Environ Microbiol. 1993 Oct;59(10):3485-7. doi: 10.1128/aem.59.10.3485-3487.1993.
Abstract
The expression at different temperatures of the lacZ gene, which is controlled by the lambda pL and pR tandem promoters and the cI857 temperature-sensitive repressor, was studied in Escherichia coli continuous cultures. At temperatures between 30 and 42 degrees C, beta-galactosidase activity behaved according to an exponential equation. By inducing a culture at a temperature within this range, predefined, nearly constant submaximal levels of gene expression and recombinant product yield can be obtained.
摘要
在大肠杆菌连续培养物中，研究了由λ pL和pR串联启动子以及cI857温度敏感阻遏物控制的lacZ基因在不同温度下的表达情况。在30至42摄氏度之间的温度下，β-半乳糖苷酶活性符合指数方程。通过在该温度范围内诱导培养，可以获得预定义的、接近恒定的亚最大水平的基因表达和重组产物产量。
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