胞内分枝杆菌α抗原基因的克隆、测序与表达以及利用聚合酶链反应快速鉴定胞内分枝杆菌
Cloning, sequencing and expression of the gene for alpha antigen from Mycobacterium intracellulare and use of PCR for the rapid identification of Mycobacterium intracellulare.
作者信息
Kitaura H, Ohara N, Matsuo T, Tasaka H, Kobayashi K, Yamada T
机构信息
School of Dentistry, Nagasaki University, Japan.
出版信息
Biochem Biophys Res Commun. 1993 Nov 15;196(3):1466-73. doi: 10.1006/bbrc.1993.2417.
The complete nucleotide sequence of alpha antigen secreted from Mycobacterium intracellulare (ATCC13950) was determined. The gene encoded 330 amino acids including 40 amino acids for signal peptide, followed by 290 amino acids for a mature protein with molecular mass 30,645 Da. The cloned gene was expressed in Escherichia coli by using an E. coli expression vector. Based on these results, the feasibility of rapid identification of M. intracellulare by two step polymerase chain reaction (PCR) was demonstrated.
测定了胞内分枝杆菌（ATCC13950）分泌的α抗原的完整核苷酸序列。该基因编码330个氨基酸，其中包括40个氨基酸的信号肽，随后是290个氨基酸的成熟蛋白，分子量为30,645 Da。通过使用大肠杆菌表达载体，将克隆的基因在大肠杆菌中进行表达。基于这些结果，证明了通过两步聚合酶链反应（PCR）快速鉴定胞内分枝杆菌的可行性。
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