Shiratori Y, Takada H, Hikiba Y, Okano K, Niwa Y, Matsumura M, Komatsu Y, Omata M
Division of Gastroenterology and Hepatology, Asahi Life Foundation, Tokyo, Japan.
Biochem Biophys Res Commun. 1993 Nov 30;197(1):319-25. doi: 10.1006/bbrc.1993.2478.
In an attempt to clarify the mechanisms of neutrophil-accumulation in the liver of alcoholics, release of KC/gro protein (intercrine cytokine family) from hepatocytes in the chronically ethanol fed rats was examined. Chemotactic activity for rat neutrophils was demonstrated in the culture supernatant of hepatocytes isolated from the ethanol fed rats, which was then inhibited in the presence of antibody against rat KC/gro protein. Immunoblot analysis revealed that the peptide reacted with antiserum against KC/gro protein was demonstrated at a molecular weight of 20-23kDa, and an amount of KC/gro protein released from the hepatocytes of the chronically ethanol fed rats was increased, as compared with that of the control rats.
为了阐明酒精性肝病患者肝脏中中性粒细胞聚集的机制，研究了长期喂食乙醇的大鼠肝细胞中KC/gro蛋白（细胞间分泌细胞因子家族）的释放情况。从喂食乙醇的大鼠分离出的肝细胞培养上清液中显示出对大鼠中性粒细胞的趋化活性，而在存在抗大鼠KC/gro蛋白抗体的情况下，这种活性受到抑制。免疫印迹分析显示，与抗血清反应的、分子量为20 - 23kDa的肽被证实存在，并且与对照大鼠相比，长期喂食乙醇的大鼠肝细胞释放的KC/gro蛋白量增加。
