Odake H, Koizumi F, Hatakeyama S, Furuta I, Nakagawa H
Department of Oral and Maxillo-Facial Surgery, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
Exp Mol Pathol. 1993 Feb;58(1):14-24. doi: 10.1006/exmp.1993.1002.
Three major neutrophil chemotactic peptides belonging to the interleukin-8 (IL-8) family were purified to homogeneity from the conditioned medium of the IL-1 beta-stimulated human gingival fibroblasts culture. On the basis of their molecular masses and NH2-terminal amino acid sequences, these three neutrophil chemotactic factors were identical to melanoma growth stimulatory activity-alpha (MGSA/gro-alpha), MGSA/gro-gamma and Ala-Val-Leu-Pro-Arg-IL-8 (AVLPR-IL-8), each belonging to the IL-8 family. It has been shown by the chemotaxis studies in vitro that the chemotactic activity of MGSA/gro-gamma is similar to that of MGSA/gro-alpha for both human and rat neutrophils, while AVLPR-IL-8 is chemotactic for human neutrophils but not for rat neutrophils. The in vitro findings were supported by the histological studies in vivo on the intradermal injection of either MGSA/gro-alpha or AVLPR-IL-8 into the back of the rats. The results obtained in the present experiment suggest that human gingival fibroblasts play an important role in gingival inflammation through the production of neutrophil chemotactic factors newly characterized as the IL-8 family in response to inflammatory chemical mediators including IL-1 beta.
从白细胞介素-1β刺激的人牙龈成纤维细胞培养的条件培养基中纯化出三种属于白细胞介素-8（IL-8）家族的主要中性粒细胞趋化肽，并使其达到均一性。根据它们的分子量和氨基末端氨基酸序列，这三种中性粒细胞趋化因子与黑素瘤生长刺激活性-α（MGSA/gro-α）、MGSA/gro-γ和丙氨酸-缬氨酸-亮氨酸-脯氨酸-精氨酸-IL-8（AVLPR-IL-8）相同，它们各自属于IL-8家族。体外趋化性研究表明，MGSA/gro-γ对人和大鼠中性粒细胞的趋化活性与MGSA/gro-α相似，而AVLPR-IL-8对人中性粒细胞有趋化作用，但对大鼠中性粒细胞没有趋化作用。体内组织学研究通过向大鼠背部皮内注射MGSA/gro-α或AVLPR-IL-8支持了体外研究结果。本实验获得的结果表明，人牙龈成纤维细胞通过产生新鉴定为IL-8家族的中性粒细胞趋化因子，在牙龈炎症中发挥重要作用，这些趋化因子是对包括IL-1β在内的炎症化学介质作出的反应。
