Prosniak M I, Veselovskaia S I, Miasnikov V A, Efremova E Iu, Potapov V K, Limborskaia S A, Sverdlov E D
Bioorg Khim. 1993 Sep;19(9):917-9.
The modified oligodeoxyribonucleotide (m5C-n2A-m5C)5 containing 5-methylcytosine and 2-aminoadenine instead of cytosine and adenine residues, respectively has been used as a hybridisation probe in DNA fingerprinting. The oligonucleotide, due to the substitutions forms more stable duplexes with complementary sequence in DNA than the corresponding nonmodified pentadecanucleotide. The comparison with its natural counterpart displays considerably increased intensity of bands in patterns obtained with modified analog. The use of such analogues can increase sensitivity and shorten time of DNA fingerprinting.
含有5-甲基胞嘧啶和2-氨基腺嘌呤分别替代胞嘧啶和腺嘌呤残基的修饰寡脱氧核糖核苷酸（m5C-n2A-m5C）5已被用作DNA指纹图谱中的杂交探针。由于这些取代，该寡核苷酸与DNA中的互补序列形成比相应未修饰的十五聚体更稳定的双链体。与天然对应物的比较显示，用修饰类似物获得的图谱中条带强度显著增加。使用此类类似物可提高DNA指纹图谱的灵敏度并缩短时间。
