[The construction of human genome YAC library and screening by hybridization of membrane high density spotted in array].
作者信息
Chai J, Gu Y
机构信息
Institute of Genetics, Fudan University, Shanghai.
出版信息
Yi Chuan Xue Bao. 1993;20(4):285-9.
PMID:8251192
Abstract
The human genomic YAC (Yeast Artificial Chromosome) library was constructed with human white blood cell and the cell line GM1 414 containing 4 X chromosome DNA using pYAC 4 as vector. Twenty thousand YAC clones were obtained with insert size of 400-1000kb. A set of YACs containing entire DMD geneDNA sequence were selected.
摘要
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