Hudson J A, Mott S J
Meat Industry Research Institute of New Zealand Inc., Hamilton.
Int J Food Microbiol. 1993 Oct;20(1):1-11. doi: 10.1016/0168-1605(93)90055-l.
A reference or type strain and a food derived-strain of the cold-tolerant pathogens Listeria monocytogenes, Aeromonas hydrophila and Yersinia enterocolitica were individually inoculated into samples of commercial pâté and incubated at 4 and 10 degrees C. The organisms were periodically estimated by presumptive counts, then values for the lag and generation times were calculated. Both strains of L. monocytogenes grew at both temperatures. The food strain of A. hydrophila grew only at 10 degrees C, and the type strain did not grow at either temperature. Similarly, the type strain of Y. enterocolitica did not grow at either temperature, whereas the food strain grew at both 4 and 10 degrees C. In some cases growth of non-test organisms may have inhibited the growth of these latter two species. The measured values of lag and generation times did not, in general, correlate well with those predicted by response surface models, taken from the literature and produced in this laboratory. It may be that the pâté contained an inhibitor that affected the growth of the organisms. The two strains of A. hydrophila and Y. enterocolitica showed significantly different growth characteristics, reinforcing the value of using a 'cocktail' of strains in growth experiments. Differences in predicted kinetic values from the models indicate that a model for any particular strain may not reflect the growth of naturally occurring contaminants of the same species.
将耐冷病原体单核细胞增生李斯特菌、嗜水气单胞菌和小肠结肠炎耶尔森菌的参考菌株或模式菌株以及食品来源菌株分别接种到市售肉酱样品中，并在4℃和10℃下培养。通过推测计数定期评估这些微生物，然后计算延迟期和代时的值。两种单核细胞增生李斯特菌菌株在这两个温度下均生长。嗜水气单胞菌的食品菌株仅在10℃下生长，而模式菌株在这两个温度下均不生长。同样，小肠结肠炎耶尔森菌的模式菌株在这两个温度下均不生长，而食品菌株在4℃和10℃下均生长。在某些情况下，非测试微生物的生长可能抑制了后两个物种的生长。延迟期和代时的测量值总体上与从文献中获取并在本实验室产生的响应面模型预测的值相关性不佳。肉酱中可能含有影响微生物生长的抑制剂。嗜水气单胞菌和小肠结肠炎耶尔森菌的两种菌株表现出显著不同的生长特性，这强化了在生长实验中使用菌株“鸡尾酒”的价值。模型预测动力学值的差异表明，针对任何特定菌株的模型可能无法反映同一物种天然存在的污染物的生长情况。
