Acuña M I, Mingot F, Davila C A
Biochim Biophys Acta. 1976 Nov 12;454(1):45-50. doi: 10.1016/0005-2787(76)90352-x.
The hypochromicity recovery of a partially denatured DNA sample when salt concentration is suddenly increased at an intermediate stage of the transition, is studied. The results of CsC1 gradient analysis, polyethylenglycol/dextran partition analysis, and the behaviour in a new thermal transition, support the view that the process is an intramolecular double chain renaturation as that induced by cooling. The degree of denaturation irreversibility is dependent on the size of DNA segments between two nicks and on the helicity value at which salt concentration-jump is performed.
研究了在转变的中间阶段盐浓度突然增加时部分变性DNA样品的减色性恢复情况。CsC1梯度分析、聚乙二醇/葡聚糖分配分析结果以及在新的热转变中的行为，支持了该过程是一种分子内双链复性的观点，如同冷却所诱导的那样。变性不可逆程度取决于两个切口之间DNA片段的大小以及进行盐浓度跃升时的螺旋值。
