小牛胸腺DNA三种卫星成分的重缔合动力学

Reassociation kinetics of three satellite components of calf thymus DNA.

作者信息

Votavová H, Sponar J

出版信息

Nucleic Acids Res. 1975 Feb;2(2):185-96. doi: 10.1093/nar/2.2.185.

DOI:10.1093/nar/2.2.185

PMID:1168341

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342825/

Abstract

Using absorption measurements the reassociation kinetics of three satellite DNA components isolated from calf thymus was studied under various conditions. A different method using CsC1 density gradient determinations particularly suited for kinetic analysis of mixtures was also used and shown to give similar results. Reassociation rate constants were corrected for mismatching during strand reassociation using data obtained by kinetic analysis of fractions of the 1.714 g/cm-3 satellite component. The values of corrected as well as uncorrected complexities were calculated and compared with results of other methods. They were shown to be compatible with the concept of sequence repetition at various levels.

摘要

利用吸收测量法，研究了从小牛胸腺中分离出的三种卫星DNA组分在不同条件下的重缔合动力学。还使用了一种特别适用于混合物动力学分析的不同方法，即CsC1密度梯度测定法，结果表明该方法能给出相似的结果。利用通过对1.714 g/cm³卫星组分的各部分进行动力学分析获得的数据，对链重缔合过程中的错配进行了校正，得到了重缔合速率常数。计算了校正和未校正的复杂度值，并与其他方法的结果进行了比较。结果表明，它们与不同水平的序列重复概念是相符的。